Digital transformation has become a strategic imperative for organizations seeking competitiveness, innovation, and operational efficiency in the digital era. Beyond technological adoption, digital transformation significantly influences organizational culture by reshaping employee behaviors, leadership styles, communication patterns, decision-making processes, and organizational values. This study examines the impact of digital transformation on organizational culture through a comprehensive review of existing literature, theoretical frameworks, and practical implementations across industries. The paper explores how digital technologies foster agility, collaboration, innovation, and data-driven decision-making while simultaneously creating challenges related to resistance to change, digital skills gaps, employee well-being, and cultural alignment. Findings indicate that successful digital transformation requires not only technological investment but also cultural adaptation and leadership commitment. Organizations that cultivate a digital-ready culture demonstrate greater innovation capacity, employee engagement, and long-term sustainability. The study concludes by proposing strategic recommendations for managing cultural transformation in increasingly digital business environments.